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Superpenetration Multi-Photon Microscopy Technique

Kilo-DM Mirror Mount
AOSLO
S-MPM Microscopy
Two-Photon Microscope
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Superpenetration Multi-Photon Microscopy was pioneered at the Cui Lab at the Janelia Farm Research Campus at the Howard Hughes Medical Institute and further developed at Boston University. The enabling component, the Kilo-S Deformable Mirror System, was then commercialized by Boston Micromachines Corporation. The Kilo-DM is an enabling component for fast and precise wavefront control. Paired with the S-driver for high-speed and low stroke operation, this system is ideal for increased depth imaging and correcting for scattering media. With this system, images of 1 µm diameter fluorescent beads through 280 µm thick mouse skull have reached image depths of about 500 µm.

Kilo-DM   S-Driver

Kilo-S-DM Specifications

 

S-Driver Specifications

  • Continuous (DM) or Segmented (SLM) surface
  • 32 actuators across a square active aperture (1020 total) or 34 actuators across a circular active aperture (952 total)
  • Stroke: 600 nm
  • Coating: Aluminum, gold or protected silver
  • Protective window with AR coating
  • Zero hysteresis
  • Fill Factor >99% (DM), 98% (SLM)
  • Sub-nm average step size
  • Surface Finish: <20 nm RMS

492-DM also available as option

 
  • DIO Interface: SFP fiber link (2.5 Gbps)
  • Latency: 22.7 µs
  • Maximum Frame Rate: 60 kHz
  • Resolution: 12 Bit
  • Average Voltage Step Size: 14 mV
  • Dimensions: 3.5” × 19” × 14” (3U Chassis for 19” Rack

The data below is from Boston University using a Kilo-SLM and high speed S-Driver for increased depth imaging through a mouse skull. For more application information on this technique, please click here.

Imaging fluorescent beads through a mouse skull

  • Mouse skull 280 µm thick
  • Glass cover slip 150 µm thick
  • Fluorescent beads, 1 µm diameter
Camera image of mouse skull Camera image (Transmission, before optimization)     Imaging camera
PMT image PMT image (reflection)
Optimized image Optical window ~20 µm

Kilo-SLM Performance Testing

Maximum update rates
  • Small displacement:  >50 kHz
  • Mid-range displacement:  >20 kHz
  • Full-range displacement: >10 kHz
MEMS SLM Segment displacement vs. frame rate

All pictures and videos courtesy Precision Engineering Research Lab (PERL) @ Boston University, Thomas Bifano, Director of the BU Photonics Center

To request more information, click here.

 
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