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S1 cortex of Thy1-YFP (H line) mice at large depth
Iterative Multi-Photon Adaptive Compensation Technique (IMPACT)
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The main scientific endeavor in confocal microscopy is to study cellular processes; however, studying these processes in living tissue is both critical and challenging to the field. Traditionally, non-invasive in vivo imaging has struggled with a fundamental problem: using enough light to illuminate a sample when using too much light can cause damage to the sample. Due to advancements in adaptive optics, confocal microscopy can now avoid this trade-off. Because adaptive optics increases not only resolution, but also signal strength and contrast level, low-light levels can now be used for clear, deep-tissue imaging in vivo.
Whole Cell - 4Pi single marker switching nanoscopy with deformable mirrors
•Work released by M. Cui of Janelia Research Campus, HHMI
•In vivo imaging of functional mouse brain through the skull
In Vivo Multiplexed Modulation AO Imaging
Functional imaging of S1 cortex with awake, head-restrained GP5.17 mouse