Iterative Multi-Photon Adaptive Compensation Technique (IMPACT)
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In Vivo Multiplexed Modulation AO Imaging
Functional imaging of S1 cortex with awake, head-restrained GP5.17 mouse
The main scientific endeavor in confocal microscopy is to study cellular processes; however, studying these processes in living tissue is both critical and challenging to the field. Traditionally, non-invasive in vivo imaging has struggled with a fundamental problem: using enough light to illuminate a sample when using too much light can cause damage to the sample. Due to advancements in adaptive optics, confocal microscopy can now avoid this trade-off. Because adaptive optics increases not only resolution, but also signal strength and contrast level, low-light levels can now be used for clear, deep-tissue imaging in vivo.
Whole Cell - 4Pi single marker switching nanoscopy with deformable mirrors
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S1 cortex of Thy1-YFP (H line) mice at large depth
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•Work released by M. Cui of Janelia Research Campus, HHMI
•In vivo imaging of functional mouse brain through the skull